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Structures and rheological properties of hen egg yolk low density lipoprotein layers spread at the air–water interface at pH 3 and 7 by Stéphanie Dauphas; Valérie Beaumal; Paul Gunning; Alan Mackie; Peter Wilde; Véronique Vié; Alain Riaublanc; Marc Anton is a Materials Science article available to read on EtoBox.
What is Structures and rheological properties of hen egg yolk low density lipoprotein layers spread at the air–water interface at pH 3 and 7 about?
Low density lipoproteins (LDL) from egg yolk have a classical structure of lipoprotein with a core of neutral lipids surrounded by a monolayer of apoproteins and phospholipids. This structure collapses during adsorption and all constituents spread at the interface. To understand better the nature of the interactions between apoproteins and lipids at the interface, we have deposited LDL at an air-water interface and analysed the isotherms during their compression on a Langmuir trough. Then, these LDL films were studied by atomic force microscopy (AFM) imaging. To identify the protein and lipid structures, we imaged films before and after lipid solubilisation by butanol. To study the interactions in the LDL films, we have varied the pH, ionic strength and used simplified model systems. We also studied the correlation between observed structures and interfacial rheology of the film. The isotherms of interfacial LDL films were similar for pH 3 and 7, but their structures observed in AFM were different. At surface pressures below the transition corresponding to the demixion of apoprotein-neutral lipid complexes, the LDL film structure was not governed by electrostatic interactions. Howe
Who reads Structures and rheological properties of hen egg yolk low density lipoprotein layers spread at the air–water interface at pH 3 and 7?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Stéphanie Dauphas; Valérie Beaumal; Paul Gunning; Alan Mackie; Peter Wilde; Véronique Vié; Alain Riaublanc; Marc Anton
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7765)
- Published
- 2007
- Language
- EN
- Field
- Materials Science (Physical Sciences)