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Mixed-ligand Modification of Polyamidoamine Dendrimers to Develop an Effective Scaffold for Maintenance of Hepatocyte Spheroids by S. Higashiyama; M. Noda; M. Kawase; K. Yagi is a Engineering article available to read on EtoBox.

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## Abstract Compared with a monolayer culture, hepatocyte spheroids are known to maintain liver function for long periods. We found that hepatocytes formed spheroids when cultured on polyamidoamine dendrimers modified with fructose. Because galactose is a ligand for the asialoglycoprotein receptor on the hepatocyte cytoplasmic membrane, it was chosen as another ligand for modification in order to maintain adhesion of spheroids for long periods. Simultaneous modification of dendrimers with fructose and galactose had a marked effect on the time length of spheroid adhesion. Suppression of apoptosis and necrosis was observed in hepatocyte spheroids cultured on a dendrimer modified with fructose and galactose (F/G dendrimer). Moreover, the hepatocyte spheroids cultured on the F/G dendrimer had higher activities of liver‐specific functions, such as urea synthesis and albumin gene expression, than did those cultured on single‐ligand‐modified dendrimers. The expression of heat shock protein (HSP) genes was examined to evaluate the stress response of cells to scaffolds. The hepatocytes cultured on the F/G dendrimer had very low expression levels of both HSP60 and HSP70 mRNAs. Thus immobiliz

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Author

S. Higashiyama; M. Noda; M. Kawase; K. Yagi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)

Published

2003

Language

EN

Field

Engineering (Physical Sciences)