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Optimization of use of UEA-1 magnetic beads for endothelial cell isolation by V. Conrad-Lapostolle; L. Bordenave; Ch. Baquey is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Optimization of use of UEA-1 magnetic beads for endothelial cell isolation about?

Most endothelial cells (EC) in the body belong to the microvasculature. Isolation and subsequent culture of these microvessel EC contributes greatly to our understanding of the heterogeneity and vascular specificity that exist between one organ site and another. However, a major obstacle is the overgrowth of contaminating cells (fibroblasts, pericytes, smooth-muscle cells) in cultures. Since 1990 the use of magnetic beads in combination with either a lectin, Ulex europaeus agglutinin-1 (UEA-1), or a monoclonal antibody has represented a powerful tool for the isolation/purification of microvessel EC. In the former case, operative conditions remain to be optimized to obtain pure cultures of EC. We have performed studies to optimize conditions of use for magnetic beads coated with UEA-1. Incubating beads with cells, the influences are studied of time, temperature, cell concentration, and number of beads per target cell for two cell types, human umbilical vein EC (HUVEC) and skin fibroblasts (HSF), either isolated or mixed. The effect of the last parameter was also checked on the behavior of cells undergoing proliferation after isolation. Results, expressed as isolation efficiency (fro

Who reads Optimization of use of UEA-1 magnetic beads for endothelial cell isolation?

Author

V. Conrad-Lapostolle; L. Bordenave; Ch. Baquey

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0742-2091)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)