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Temperature‐dependent expression of conjugation pili by IncM plasmid‐harbouring bacteria: Identification of plasmid‐encoded regulatory functions by Erhard Tietze; Helmut Tschäpe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A 1,3 kb DNA fragment of the IncM plasmid R446, if cloned in a multicopy plasmid, inhibits __in trans__ the expression of conjugation pili by IncM plasmid‐harbouring host bacteria as indicated by their insensitivity to the IncM pilus‐dependent bacteriophage φM (Iml, insensitivity to φM‐mediated lysis). Determination of the nucleotide sequence of this DNA fragment, the introduction of deletions and the analysis of transposon insertions reveal two determinants, __iml__A and __iml__B, responsible for the Iml phenotype. A stretch of 80 bp of DNA containing __iml__A and about 450 bp of adjacent DNA comprising __iml__B, together, bring about inhibition of the typical expression of conjugation pili at 30 °C. The introduction of a transposable promoter probe and the construction of respective __lac__Z fusions indicate transcription of complementary strands __in vivo__ overlapping in the region comprising __iml__A and __iml__B. Moreover, the expression of reporter genes discloses temperature‐dependent transcription of the __iml__A‐__iml__B‐region in one direction. A particular subfragment of the 1.3 kb IncM plasmid‐derived DNA does not inhibit conjugation pilus expression at 30

Who reads Temperature‐dependent expression of conjugation pili by IncM plasmid‐harbouring bacteria: Identification of plasmid‐encoded regulatory functions?

Author

Erhard Tietze; Helmut Tschäpe

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)