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Expression of retinol‐binding protein messenger RNA and retinoic acid receptors in preattachment bovine embryos by M. Mohan; J.R. Malayer; R.D. Geisert; G.L. Morgan is a Medicine article available to read on EtoBox.

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## Abstract In cattle, retinoic acid (RA) has been indirectly associated with developmental potential of the embryo. RA is transported by retinol‐binding protein (RBP) and actions of RA are mediated by several subtypes of nuclear retinoic acid receptors (RAR). Bovine embryos, produced in vitro from oocytes harvested from ovaries collected at a local abattoir, were frozen in liquid nitrogen at the oocyte, 2‐, 4‐, 8‐, 16 to 20‐cell, morula, blastocyst, and hatched blastocyst stages. Employing reverse transcription polymerase chain reaction (RT‐PCR) we investigated mRNA expression for RBP, RARα, RARβ, RARγ, and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). Total RNA was extracted from 25 pooled embryos at each stage and RT‐PCR analysis was repeated thrice. GAPDH transcript was detected in all stages. Transcripts for RBP, RARα, and RARγ were also detected in all stages from the oocyte through to the hatched blastocyst. Expression of RARβ was not detected at any stage. Whole‐mount immunohistochemistry was performed with intact and hatched blastocysts using polyclonal antibodies against RARα and RARγ2 to investigate if these embryonic mRNAs were translated to the mature protein. Stro

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Author

M. Mohan; J.R. Malayer; R.D. Geisert; G.L. Morgan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)

Published

2001

Language

EN

Field

Medicine (Life Sciences)