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Liquid Chromatographic–atmospheric Pressure Chemical Ionization Mass Spectrometric Analysis of Opiates and Metabolites in Rat Urine After Inhalation of Opium by R Kikura-Hanajiri; N Kaniwa; M Ishibashi; Y Makino; S Kojima is a Chemistry article available to read on EtoBox.

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To examine the urinary excretion of opiates and their metabolites following inhalation exposure of rats to opium, analytical procedures for the simultaneous determination of the compounds in opium, the vapor derived by the volatilization of opium and the urine of rats exposed to the opium vapor were developed using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS). Seven compounds were determined in the opium, namely morphine, codeine, thebaine, noscapine, papaverine, meconic acid and meconin. All seven were extracted with 2.5% acetic acid solution and subjected to LC-APCI-MS analysis. The separation was performed on an ODS column in acetonitrile-50 mM ammonium formate buffer (pH 3.0) using a linear gradient program and quantitative analysis was carried out in the selected ion monitoring mode ([M+H](+)). For the analysis of the volatilization of opium, the opium (1 g) was added to a glass pipe, which was then heated at 300 degrees C for 20 min. Negative pressure (air flow-rate; 300 ml/min) was used to draw the vapor through a series of glass wool and methanol traps. The total amount of each compound in the vapor was estimated by measureme

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Author

R Kikura-Hanajiri; N Kaniwa; M Ishibashi; Y Makino; S Kojima

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)