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Production of Lentiviral Vectors with Enhanced Efficiency to Target Dendritic Cells by Attenuating Mannosidase Activity of Mammalian Cells by April Tai; Steven Froelich; Kye-Il Joo; Pin Wang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Production of Lentiviral Vectors with Enhanced Efficiency to Target Dendritic Cells by Attenuating Mannosidase Activity of Mammalian Cells about?

## Background Dendritic cells (DCs) are antigen-presenting immune cells that interact with T cells and have been widely studied for vaccine applications. To achieve this, DCs can be manipulated by lentiviral vectors (LVs) to express antigens to stimulate the desired antigen-specific T cell response, which gives this approach great potential to fight diseases such as cancers, HIV, and autoimmune diseases. Previously we showed that LVs enveloped with an engineered Sindbis virus glycoprotein (SVGmu) could target DCs through a specific interaction with DC-SIGN, a surface molecule predominantly expressed by DCs. We hypothesized that SVGmu interacts with DC-SIGN in a mannose-dependent manner, and that an increase in high-mannose structures on the glycoprotein surface could result in higher targeting efficiencies of LVs towards DCs. It is known that 1-deoxymannojirimycin (DMJ) can inhibit mannosidase, which is an enzyme that removes high-mannose structures during the glycosylation process. Thus, we investigated the possibility of generating LVs with enhanced capability to modify DCs by supplying DMJ during vector production. ## Results Through western blot analysis and binding tests, we w

Who reads Production of Lentiviral Vectors with Enhanced Efficiency to Target Dendritic Cells by Attenuating Mannosidase Activity of Mammalian Cells?

Author

April Tai; Steven Froelich; Kye-Il Joo; Pin Wang

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2007-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1754-1611)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)