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Pyrosequencing is a DNA sequencing technique that emerged in January 1986, based on monitoring the activity of DNA polymerase during nucleotide incorporation. It is a PCR-based, enzymatic method that allows for short to medium range sequencing of up to 30-50 nucleotides and provides quantitative data on mutation percentages. The technique has been notably applied in identifying mutations such as the IDH1 mutation in gliomas.

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