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A Multi-enzyme Cascade Reaction for the Production of 6-Hydroxyhexanoic Acid by Vishnu S.T. Srinivasamurthy; Dominique Böttcher; Uwe T. Bornscheuer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Multi-enzyme Cascade Reaction for the Production of 6-Hydroxyhexanoic Acid about?

Multi-enzyme cascade reactions capture the essence of nature's efficiency by increasing the productivity of a process. Here we describe one such three-enzyme cascade for the synthesis of 6-hydroxyhexanoic acid. Whole cells of Escherichia coli co-expressing an alcohol dehydrogenase and a Baeyer-Villiger monooxygenase (CHMO) for internal cofactor regeneration were used without the supply of external NADPH or NADP+. The product inhibition caused by the ε-caprolactone formed by the CHMO was overcome by the use of lipase CAL-B for in situ conversion into 6-hydroxyhexanoic acid. A stirred tank reactor under fed-batch mode was chosen for efficient catalysis. By using this setup, a product titre of >20 g L-1 was achieved in a 500 mL scale with an isolated yield of 81% 6-hydroxyhexanoic acid.

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Author

Vishnu S.T. Srinivasamurthy; Dominique Böttcher; Uwe T. Bornscheuer

Publisher

Walter de Gruyter GmbH

Published

2019

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)