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On‐chip Solid‐phase Extraction Pre‐concentration/focusing Substrates Coupled to Atmospheric Pressure Matrix‐assisted Laser Desorption/ionization Ion Trap Mass Spectrometry for High Sensitivity Biomolecule Analysis by Arti Navare; Marcela Nouzova; Fernando G. Noriega; Salvador Hernández‐Martínez; Christoph Menzel; Facundo M. Fernández is a Chemistry article available to read on EtoBox.

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## Abstract Atmospheric pressure matrix‐assisted laser desorption/ionization (AP‐MALDI) has proven a convenient and rapid method for ion production in the mass spectrometric (MS) analysis of biomolecules. AP‐MALDI and electrospray ionization (ESI) sources are easily interchangeable in most mass spectrometers. However, AP‐MALDI suffers from less‐than‐optimal sensitivity due to ion losses during transport from the atmosphere into the vacuum of the mass spectrometer. Here, we study the signal‐to‐noise ratio (S/N) gains observed when an on‐chip dynamic pre‐concentration/focusing approach is coupled to AP‐MALDI for the MS analysis of neuropeptides and protein digests. It was found that, in comparison with conventional AP‐MALDI targets, focusing targets showed (1) a sensitivity enhancement of approximately two orders of magnitude with S/N gains of 200–900 for hydrophobic substrates, and 150–400 for weak cation‐exchange (WCX) substrates; (2) improved detection limits as low as 5 fmol/μL for standard peptides; (3) significantly reduced matrix background; and (4) higher inter‐day reproducibility. The improved sensitivity allowed successful tandem mass spectrometric (MS/MS) sequencing of dil

Who reads On‐chip Solid‐phase Extraction Pre‐concentration/focusing Substrates Coupled to Atmospheric Pressure Matrix‐assisted Laser Desorption/ionization Ion Trap Mass Spectrometry for High Sensitivity Biomolecule Analysis?

Author

Arti Navare; Marcela Nouzova; Fernando G. Noriega; Salvador Hernández‐Martínez; Christoph Menzel; Facundo M. Fernández

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)