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Organization and structure of the 1-aminocyclopropane-1-carboxylate oxidase gene family from Petunia hybrida by Xiaoyan Tang; Hong Wang; Amanda S. Brandt; William R. Woodson is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Organization and structure of the 1-aminocyclopropane-1-carboxylate oxidase gene family from Petunia hybrida about?
In this paper we present the structural analysis of the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family from Petunia hybrida. Southern blot analysis and restriction endonuclease mapping showed that two cloned regions of the petunia genome contained sequences highly homologous to a previously isolated ACC oxidase cDNA clone. Nucleotide sequencing of these two regions of the genome showed that each contained two tandemly arranged genes designated ACO1, AC02, AC03 and AC04. Comparison of the nucleotide sequences of the cloned genomic regions with the cDNA clone pPHEFE indicated that ACO1 encoded the transcript in 4 exons interrupted by 3 introns. The other three members of the petunia ACC oxidase gene family shared identical intron numbers and positions with AC01 and their exons were greater than 80 ~o homologous. Nucleotide substitutions and deletions in the A C02 gene indicate that it likely represents a pseudogene. Overall homology between AC01 and AC02 indicates that this gene cluster arose by a more recent duplication event than the gene duplication giving rise to the AC03 and AC04 cluster. The 5-flanking sequences share little overall homology between members of this
Who reads Organization and structure of the 1-aminocyclopropane-1-carboxylate oxidase gene family from Petunia hybrida?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Xiaoyan Tang; Hong Wang; Amanda S. Brandt; William R. Woodson
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1993
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)