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Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus by Marina N. Vassylyeva; Jookyung Lee; Shun-ichi Sekine; Oleg Laptenko; Seiki Kuramitsu; Takehiko Shibata; Yorinao Inoue; Sergei Borukhov; Dmitry G. Vassylyev; Shigeyuki Yokoyama is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus about?
RNA polymerase holoenzyme from Thermus thermophilus, consisting of six protein subunits (alpha(2), beta, beta', omega and sigma(70)) and having a total molecular mass of about 450 kDa, was purified and crystallized by the hanging-drop vapour-diffusion technique under mild near-physiological conditions. The crystals diffract beyond 3 A resolution. Careful analysis of diffraction data revealed that the crystals belong to space group P3(2), with unit-cell parameters a = b = 236.35, c = 249.04 A, and have perfect twinning along the threefold axis. A complete data set at 3 A resolution was collected and an unambiguous molecular-replacement solution was found using the structure of T. aquaticus RNA polymerase core enzyme as a search model. The refinement of structure and model building of the sigma(70) subunit is now in progress.
Who reads Purification, crystallization and initial crystallographic analysis of RNA polymerase holoenzyme from Thermus thermophilus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Marina N. Vassylyeva; Jookyung Lee; Shun-ichi Sekine; Oleg Laptenko; Seiki Kuramitsu; Takehiko Shibata; Yorinao Inoue; Sergei Borukhov; Dmitry G. Vassylyev; Shigeyuki Yokoyama
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)