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Modulation of Fatty Acid-Related Genes in the Response of H9c2 Cardiac Cells to Palmitate and n-3 Polyunsaturated Fatty Acids by Cetrullo, Silvia; DâAdamo, Stefania; Panichi, Veronica; Borzì, Rosa Maria; Pignatti, Carla; Flamigni, Flavio is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Modulation of Fatty Acid-Related Genes in the Response of H9c2 Cardiac Cells to Palmitate and n-3 Polyunsaturated Fatty Acids about?

While high levels of saturated fatty acids are associated with impairment of cardiovascular functions, n-3 polyunsaturated fatty acids (PUFAs) have been shown to exert protective effects. However the molecular mechanisms underlying this evidence are not completely understood. In the present study we have used rat H9c2 ventricular cardiomyoblasts as a cellular model of lipotoxicity to highlight the effects of palmitate, a saturated fatty acid, on genetic and epigenetic modulation of fatty acid metabolism and fate, and the ability of PUFAs, eicosapentaenoic acid, and docosahexaenoic acid, to contrast the actions that may contribute to cardiac dysfunction and remodeling. Treatment with a high dose of palmitate provoked mitochondrial depolarization, apoptosis, and hypertrophy of cardiomyoblasts. Palmitate also enhanced the mRNA levels of sterol regulatory element-binding proteins (SREBPs), a family of master transcription factors for lipogenesis, and it favored the expression of genes encoding key enzymes that metabolically activate palmitate and commit it to biosynthetic pathways. Moreover, miR-33a, a highly conserved microRNA embedded in an intronic sequence of the SREBP2 gene, was c

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Author

Cetrullo, Silvia; DâAdamo, Stefania; Panichi, Veronica; Borzì, Rosa Maria; Pignatti, Carla; Flamigni, Flavio

Publisher

Multidisciplinary Digital Publishing Institute (MDPI) (ISSN 2073-4409)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)