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Microarray-based Kinetic Colorimetric Detection for Quantitative Multiplex Protein Phosphorylation Analysis by Pavlo Holenya; Igor Kitanovic; Florian Heigwer; Stefan Wölfl is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Microarray-based Kinetic Colorimetric Detection for Quantitative Multiplex Protein Phosphorylation Analysis about?

## Abstract Commonly used colorimetric detection applied to protein microarrays with enzymatic signal amplification leads to non‐linear signal production upon increase in analyte concentration, thereby considerably limiting the range and accuracy of quantitative readout interpretation. To extend the detection range, we developed a kinetic colorimetric detection protocol for the analysis of ELISA microarrays designed to measure multiple phosphorylated proteins using the platforms ArrayTubeTM and ArrayStripTM. With our novel quantification approach, microarrays were calibrated over a broad concentration range spanning four orders of magnitude of analyte concentration with picomolar threshold. We used this design for the simultaneous quantitative measurement of 15 phosphorylated proteins on a single chip.

Who reads Microarray-based Kinetic Colorimetric Detection for Quantitative Multiplex Protein Phosphorylation Analysis?

Author

Pavlo Holenya; Igor Kitanovic; Florian Heigwer; Stefan Wölfl

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)