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Phosphatase 2A is involved in endothelial cell microtubule remodeling and barrier regulation by Krisztina Tar; Anna A. Birukova; Csilla Csortos; Éva Bakó; Joe G.N. Garcia; Alexander D. Verin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract We have recently shown that microtubule (MT) inhibitor, nocodazole (2–5 μM) significantly increases endothelial cells (EC) actomyosin contraction and permeability indicating the importance of MT in maintaining the EC barrier (Verin et al. [2001]: Cell Mol Physiol 281:L565–L574). Okadaic acid (OA, 2–5 nM), a powerful inhibitor of protein phosphatase 2A (PP2A), significantly potentiates the effect of submaximal concentrations of nocodazole (50–200 nM) on transendothelial electrical resistance (TER) suggesting the involvement of PP2A activity in the MT‐mediated EC barrier regulation. Immunofluorescent staining of EC revealed that in control cells PP2A distributes in a pattern similar to MT. Consistent with these results, we demonstrated that significant amounts of PP2A were present in MT‐enriched EC fractions indicating tight association of PP2A with MT in endothelium. Treatment of EC with OA leads to disappearance of MT‐like PP2A staining suggesting dissociation of PP2A from the MT network. Next, we examined the effect of PP2A inhibition on phosphorylation status of MT‐associated protein tau, which in its unphosphorylated form promotes MT assembly. OA caused significant i

Who reads Phosphatase 2A is involved in endothelial cell microtubule remodeling and barrier regulation?

Author

Krisztina Tar; Anna A. Birukova; Csilla Csortos; Éva Bakó; Joe G.N. Garcia; Alexander D. Verin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)