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The Extracellular Calcium‐Sensing Receptor Is Expressed in Rat Microglia and Modulates an Outward K^+^ Channel by Naibedya Chattopadhyay; Chianping Ye; Toru Yamaguchi; Masamichi Nakai; Olga Kifor; Peter M. Vassilev; Robert N. Nishimura; Edward M. Brown is a Neuroscience article available to read on EtoBox.
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Abstract: The calcium‐sensing receptor (CaR) is a G protein‐coupled receptor that “senses” extracellular calcium ions (Ca^2+^o) as an extracellular first messenger. In this report, we have shown that the CaR is expressed in primary cultures of microglial cells derived from rat brain as assessed by RT‐PCR using four CaR‐specific primer pairs followed by sequencing of the amplified products, by northern blot analysis using a CaR‐specific probe, as well as by immunocytochemistry and western analysis utilizing a specific polyclonal anti‐CaR antiserum. In addition, raising Ca^2+^, from 0.75 to 3.0 mM or addition of the polycationic CaR agonist neomycin or a “calcimimetic” CaR activator (R‐467; NPS Pharmaceuticals) increased the open state probability (Po) of a Ca^+^‐activated Kf channel having a unitary conductance of 84 ± 4 pS, indicating that the channel is modulated by the CaR. Therefore, our data strongly suggest that a functional CaR is expressed in cultured rat microglia, similar to that in parathyroid gland and kidney, which could potentially play an important role(s) in regulating microglial function.
Who reads The Extracellular Calcium‐Sensing Receptor Is Expressed in Rat Microglia and Modulates an Outward K^+^ Channel?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Naibedya Chattopadhyay; Chianping Ye; Toru Yamaguchi; Masamichi Nakai; Olga Kifor; Peter M. Vassilev; Robert N. Nishimura; Edward M. Brown
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1999
- Language
- EN
- Field
- Neuroscience (Life Sciences)