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Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater by Yumeng Chen; Meng Wang; Wei Wang; Yi Zeng; Xingjia Fan; Maobing Tu; Yushu Ma; Dongzhi Wei is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater about?

N, N-dimethylformamide is frequently present in industrial wastewater and is environmentally detrimental. The current study aims to assess the utilization and biodegradation of N, N-dimethylformamide-containing wastewater to lessen the associated environmental load. Results show that addition of wastewater containing N, N-dimethylformamide to Trichoderma reesei fermentation media enhances cellulase production and facilitates cellulose hydrolysis. However, N, N-dimethylformamide is a cellulase enhancer that is not degraded during cellulase production in T. reesei fermentation and is retained in the N, Ndimethylformamide-enhanced cellulase solution. Indeed, the cellulosic sugar solution generated via lignocellulose hydrolysis with N, N-dimethylformamide-enhanced cellulase retains N, N-dimethylformamide. We further identified three core enzyme modulesN, N-dimethylformamidase, dimethylamine dehydrogenase, and methylamine dehydrogenase enzymewhich were inserted into Escherichia coli to develop metabolically engineered strains. These strains degraded N, N-dimethylformamide and produced succinate using N, N-dimethylformamide-enhanced cellulosic sugar as the substrate. The platform descr

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Author

Yumeng Chen; Meng Wang; Wei Wang; Yi Zeng; Xingjia Fan; Maobing Tu; Yushu Ma; Dongzhi Wei

Published

2022

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)