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Chloromethane:tetrahydrofolate methyl transfer by two proteins from Methylobacterium chloromethanicum strain CM4 by Alex Studer; Erhard Stupperich; Stéphane Vuilleumier; Thomas Leisinger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Chloromethane:tetrahydrofolate methyl transfer by two proteins from Methylobacterium chloromethanicum strain CM4 about?

The __cmuA__ and __cmuB__ genes are required for growth of __Methylobacterium chloromethanicum__ strain CM4 with chloromethane as the sole carbon source. While CmuB was previously shown to possess methylcobalamin:tetrahydrofolate methyltransferase activity, sequence analysis indicated that CmuA represented a novel and so far unique two‐domain methyltransferase/corrinoid‐binding protein involved in methyl transfer from chloromethane to a corrin moiety. CmuA was purified from wild‐type __M. chloromethanicum__ strain CM4 and characterized as a monomeric, cobalt‐containing and zinc‐containing enzyme of molecular mass 67 kDa with a bound vitamin B~12~ cofactor. In combination, CmuA and CmuB proteins catalyze the __in vitro__ transfer of the methyl group of chloromethane to tetrahydrofolate, thus affording a direct link between chloromethane dehalogenation and core C1 metabolism of __Methylobacterium__. Chloromethane dehalogenase activity __in vitro__ is limited by CmuB, as formation of methyltetrahydrofolate from chloromethane displays apparent Michaelis–Menten kinetics with respect to methylated CmuA, with an apparent __K__~m~ of 0.27 μm and a __V__~max~ of 0.45 U·mg^−1^. This contrast

Who reads Chloromethane:tetrahydrofolate methyl transfer by two proteins from Methylobacterium chloromethanicum strain CM4?

Author

Alex Studer; Erhard Stupperich; Stéphane Vuilleumier; Thomas Leisinger

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Physical Sciences)