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Crystalline beef insulin was reacted under dilution conditions with 1.06 equivalents of adipic acid bis(p-nitrophenyl) ester in dimethylsulfoxide/triethylamine. Intramolecularly crosslinked, monomeric N aAl ,/V eB29 -adipoylinsulin was isolated in homogeneous form by gel filtration of the reaction products, followed by ion exchange chromatography at pH 3 and pH 8.4. The yield was 13.5%. The CD-spectrum of this insulin derivative at pH 7.9 resembles the spectrum of insulin. The hypoglycaemic activity in rats is 42%, the biological activity in the isolated fat cell assay 5 % and the immuno reactivity 5-11%, based on insulin with 25 iU/mg. Small spherical or needlelike crystals could be obtained from citrate buffer containing zinc ions. Darstellung von N otM 9 N eB29 -Adipoylifisulift i einem Zusammenfassung: Kristallisiertes Rinderinsulin wurde unter Verdünnungsbedingungen in Dimethylsulfoxid/Triäthylamin mit 1,06 Äquivalenten Adipinsäure-bisQ?-nitrophenyl)ester umgesetzt. Aus den Reaktionsprodukten wurde durch Gelfiltration, gefolgt von lonenaustauschchromatographie bei pH 3 und 8,4 in 13,5proz. Ausbeute das intramolekular vernetzte, monomere yv aAI ,W sB29 -Adipoylinsulin in einhei

Author

Dietrich Brandenburg

Publisher

Walter de Gruyter GmbH

Published

1972

Language

EN