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Transformation by inorganic arsenic compounds of normal Syrian hamster embryo cells into a neoplastic state in which they become anchorage‐independent and cause tumors in newborn hamsters by Masayuki Takahashi; J. Carl Barrett; Takeki Tsutsui is a Medicine article available to read on EtoBox.

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## Abstract Arsenic is a known human carcinogen, but little evidence exists for its carcinogenicity in animals. In order to investigate the ability of inorganic arsenics to transform normal cells into a neoplastic state, mass cultures of normal, diploid Syrian hamster embryo (SHE) cells exposed to various concentrations of sodium arsenite or sodium arsenate for 48 hr were continually passaged and tested for neoplastic transformation, as determined by anchorage‐independent growth in semisolid agar and tumorigenicity in newborn hamsters. Twenty‐one of 22 (96%) untreated, control cultures senesced by 20 passages. While 1 culture escaped senescence, it did not acquire the ability to either grow in semisolid agar or form tumors in animals. Ten of 14 (71%) cultures exposed to sodium arsenite or sodium arsenate escaped senescence. Nine of the 10 (90%) arsenic‐treated immortal cultures acquired the anchorage‐independent phenotype. Five of 5 anchorage‐independent cultures examined were tumorigenic. Two of 3 morphologically transformed colonies induced by sodium arsenate also acquired the ability to grow in semisolid agar when isolated. Amplification of the c‐__myc__ or c‐Ha‐__ras__ oncogene

Who reads Transformation by inorganic arsenic compounds of normal Syrian hamster embryo cells into a neoplastic state in which they become anchorage‐independent and cause tumors in newborn hamsters?

Author

Masayuki Takahashi; J. Carl Barrett; Takeki Tsutsui

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2002

Language

EN

Field

Medicine (Health Sciences)