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Stationary Organotypic Cultures of Oxytocin and Vasopressin Magnocellular Neurones from Rat and Mouse Hypothalamus by House; Thomas; Kusano; Gainer is a Neuroscience article available to read on EtoBox.

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Rat and mouse hypothalami from postnatal animals containing highly differentiated neurones survive very well in long‐term (>15 days __in vitro____,__ DIV) stationary organotypic cultures. Magnocellular oxytocin (OT) and vasopressin (VP) neurones are present in identifiable paraventricular (PVN), supraoptic (SON) and accessory (ACC) nuclei in these cultures. After 15 DIV in standard medium immunocytochemistry revealed 427±63 OT cells and 217±27 VP cells per cultured rat hypothalamus, and 380±72 OT cells and 622±91 VP cells per cultured mouse hypothalamus. Following a 7‐day adaptation period in standard culture medium containing serum, the rat slice‐explants survived very well after subsequent transfer to defined, serum‐ free media (SFM) for an additional 8 days. The number of OT cells surviving in SFM was 612±147 OT cells per cultured rat hypothalamus. Only 0.5% of the magnocellular OT and VP neurones in the cultures appeared to express both peptides. Experiments on c‐__fos__gene expression in these cultures showed that while only 12% of the magnocellular OT and VP neurones contained barely detectable Fos protein in their nuclei under control conditions, potassium depolarization of

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Author

House; Thomas; Kusano; Gainer

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-8194)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)