Can I read Expression and Secretion of a Lytic Polysaccharide Monooxygenase by a Fast-growing Cyanobacterium on EtoBox?

Expression and Secretion of a Lytic Polysaccharide Monooxygenase by a Fast-growing Cyanobacterium by Russo, D. A.; Zedler, J. A. Z.; Wittmann, D. N.; Möllers, B.; Singh, R. K.; Batth, T. S.; van Oort, B.; Olsen, J. V.; Bjerrum, M. J.; Jensen, P. E. is a Engineering article available to read on EtoBox.

What is Expression and Secretion of a Lytic Polysaccharide Monooxygenase by a Fast-growing Cyanobacterium about?

## Background: Cyanobacteria have the potential to become next-generation cell factories due to their ability to use co, light and inorganic nutrients to produce a range of biomolecules of commercial interest. utex 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. to establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant aa10a, a lytic polysaccharide monooxygenase from the gram-positive bacterium ## Results: Two variations of aa10a were successfully expressed in utex 2973: one containing the native n-terminal, sec-targeted, signal peptide and a second with a tat-targeted signal peptide from the trimethylamine--oxide reductase (tora). although the tora signal peptide correctly targeted the protein to the plasma membrane, the majority of the tora-aa10a was found unprocessed in the plasma membrane with a small fraction of the mature protein ultimately translocated to the periplasm. the native sec signal peptide allowed for efficient secretion of aa10a into the medium with virtually no protein

Who reads Expression and Secretion of a Lytic Polysaccharide Monooxygenase by a Fast-growing Cyanobacterium?

Author

Russo, D. A.; Zedler, J. A. Z.; Wittmann, D. N.; Möllers, B.; Singh, R. K.; Batth, T. S.; van Oort, B.; Olsen, J. V.; Bjerrum, M. J.; Jensen, P. E.

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central; Springer Science and Business Media LLC (ISSN 1754-6834)

Published

2019

Language

EN

Field

Engineering (Physical Sciences)