About this document
DNA Footprinting: Method and Applications by Nadia Khan is a document available to read on EtoBox.
DNA footprinting is a technique used to investigate the sequence specificity of DNA-binding proteins. It works by using cleavage agents that cut DNA randomly except where a protein is bound, leaving a "footprint" of protected regions. Originally developed using radiolabels and gels, it has advanced to use fluorescent labeling and capillary electrophoresis detection. It can study protein-DNA interactions in vitro and in vivo, and quantify binding strengths through varying protein concentrations.
- Author
- Nadia Khan
- Language
- EN