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Identification ALDH Expressing Cancer Stem Cells Using ALDEFLUOR by Izadora Lorrany is a document available to read on EtoBox.
What is Identification ALDH Expressing Cancer Stem Cells Using ALDEFLUOR about?
This technical bulletin outlines an optimized protocol for identifying ALDH-expressing cancer stem cells using the ALDEFLUOR® assay in human breast cancer cell lines MCF7 and SKBR3. The protocol details the incubation times and conditions necessary for optimal ALDH staining, emphasizing that SKBR3 cells serve as a positive control while MCF7 cells act as a negative control. Additionally, the document highlights the importance of proper sample handling and flow cytometry analysis for accurate results.
- Author
- Izadora Lorrany
- Language
- EN