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Can I read E‐selectin Expression Induced by Pancreas‐carcinoma‐derived Interleukin‐1α Results in Enhanced Adhesion of Pancreas‐carcinoma Cells to Endothelial Cells on EtoBox?
E‐selectin Expression Induced by Pancreas‐carcinoma‐derived Interleukin‐1α Results in Enhanced Adhesion of Pancreas‐carcinoma Cells to Endothelial Cells by Mitsuhito Kaji; Hiroshi Ishikura; Takashi Kishimoto; Makoto Omi; Akihiro Ishizu; Chisa Kimura; Toshiyuki Takahashi; Hiroyuki Kato; Takashi Yoshiki is a Medicine article available to read on EtoBox.
What is E‐selectin Expression Induced by Pancreas‐carcinoma‐derived Interleukin‐1α Results in Enhanced Adhesion of Pancreas‐carcinoma Cells to Endothelial Cells about?
## Abstract Cellular adhesion of sialyl‐Lewis‐a(SLe^a^)‐positive pancreas carcinoma to endothelial cells (EC) is augmented by activation of EC via up‐regulated E‐selectin expression on EC. Co‐cultivation of pancreas‐carcinoma cells, PCI‐24, with human umbilical‐vein endothelial cells (HUVEC) for 5 hr at the PCI‐to‐HUVEC ratio of 1:10 induced E‐selectin expression on the endothelial‐cell surface, augmenting SLe^a^‐positive pancreas‐carcinoma cell attachment with HUVEC. Culture supernatants of 6 tested pancreas‐carcinoma cell lines contained soluble, E‐selectin‐inducing factor(s). The E‐selectin‐inducing effect by the supernatants was blocked by the protein‐kinase‐C inhibitor, H7. Antibodies against SLe^a^ and E‐selectin but not SLe^x^ or ICAM‐I blocked the increased pancreas‐carcinoma‐to‐endothelial attachment. Paraformaldehyde(PFA)‐fixed PCI‐24 cells also induced E‐selectin on vascular endothelial cells upon direct contact with endothelial cells, indicating the presence of a membrane‐bound form. The 6 pancreas‐carcinoma lines all produced IL‐1α mRNA and protein but not IL‐1β or TNF‐α protein and/or mRNA Absorption of IL‐lα from the supernatants by IL‐lα‐specific antibody almost com
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- Author
- Mitsuhito Kaji; Hiroshi Ishikura; Takashi Kishimoto; Makoto Omi; Akihiro Ishizu; Chisa Kimura; Toshiyuki Takahashi; Hiroyuki Kato; Takashi Yoshiki
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1995
- Language
- EN
- Field
- Medicine (Health Sciences)