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An acquired acyltransferase promotes Klebsiella pneumoniae ST258 respiratory infection by Danielle Ahn; Gitanjali Bhushan; Thomas H. McConville; Medini K. Annavajhala; Rajesh Kumar Soni; Tania Wong Fok Lung; Casey E. Hofstaedter; Shivang S. Shah; Alexander M. Chong; Victor G. Castano; Robert K. Ernst; Anne-Catrin Uhlemann; Alice Prince is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An acquired acyltransferase promotes Klebsiella pneumoniae ST258 respiratory infection about?

## SUMMARY __Klebsiella pneumoniae__ ST258 is a human pathogen associated with poor outcomes worldwide. We identify a member of the acyltransferase superfamily 3 (__atf3__), enriched within the ST258 clade, that provides a major competitive advantage for the proliferation of these organisms __in vivo__. Comparison of a wild-type ST258 strain (KP35) and a Δ__atf3__ isogenic mutant generated by CRISPR-Cas9 targeting reveals greater NADH:ubiquinone oxidoreductase transcription and ATP generation, fueled by increased glycolysis. The acquisition of __atf3__ induces changes in the bacterial acetylome, promoting lysine acetylation of multiple proteins involved in central metabolism, specifically Zwf (glucose-6 phosphate dehydrogenase). The __atf3__-mediated metabolic boost leads to greater consumption of glucose in the host airway and increased bacterial burden in the lung, independent of cytokine levels and immune cell recruitment. Acquisition of this acyltransferase enhances fitness of a __K. pneumoniae__ ST258 isolate and may contribute to the success of this clonal complex as a healthcare-associated pathogen.

Who reads An acquired acyltransferase promotes Klebsiella pneumoniae ST258 respiratory infection?

Author

Danielle Ahn; Gitanjali Bhushan; Thomas H. McConville; Medini K. Annavajhala; Rajesh Kumar Soni; Tania Wong Fok Lung; Casey E. Hofstaedter; Shivang S. Shah; Alexander M. Chong; Victor G. Castano; Robert K. Ernst; Anne-Catrin Uhlemann; Alice Prince

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)