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Simultaneous determination of cinnamaldehyde, eugenol and piperine by HPTLC densitometric method by C. L. Gopu; Suyog Aher; Hiral Mehta; A. R. Paradkar; K. R. Mahadik is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract An HPTLC densitometric method for the simultaneous determination of cinnamaldehyde and eugenol as well as trace amounts of piperine in pepper‐contaminated cinnamon was developed. The applicability of the method was tested with cinnamon bark powder adulterated with pepper powder, cinnamon oil, clove powder, clove oil and a commercial preparation containing cinnamaldehyde and eugenol. The method was validated for specificity, precision, accuracy and robustness. The method was found to be precise for different concentrations of cinnamaldehyde, eugenol and piperine. The accuracy of the method was checked by conducting a recovery study at three different levels. The linearity was found to be in the ranges 52.54–735.56, 533.2–8531.2 and 50–300 ng/spot, respectively, with correlation coefficients of 0.9985 ± 0.04, 0.9982 ± 0.06 and 0.9937 ± 0.11 for cinnamaldehyde, eugenol and piperine. Copyright © 2007 John Wiley & Sons, Ltd.

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Author

C. L. Gopu; Suyog Aher; Hiral Mehta; A. R. Paradkar; K. R. Mahadik

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)