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Two-wavelength MAD phasing and radiation damage: a case study by Ana González; Frank von Delft; Robert C. Liddington; Constantina Bakolitsa is a Physics and Astronomy article available to read on EtoBox.

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Radiation damage affects MAD experiments in two ways: (i) increased absorption by the crystal at the wavelengths of interest for the experiment results in faster crystal deterioration; (ii) lack of isomorphism induced by radiation damage causes problems when scaling and merging data at different wavelengths and can prevent accurate measurement of anomalous and dispersive differences. In an attempt to overcome these problems in the case of radiation-sensitive crystals of vinculin, two-wavelength MAD data were collected at the Se absorption-edge inflection and at high-energy remote wavelengths. Although this strategy resulted in a lower total absorbed dose compared with a standard three-wavelength experiment using the peak wavelength, an increase in the unit-cell volume and other effects attributable to radiation damage were still observed. In an effort to extract the maximum information available from the data, different data-processing and scaling procedures were compared. Scaling approaches involving local scaling of unmerged reflections were consistently successful and most ordered Se sites could be located. Subsequent use of these sites for phasing resulted in an interpretable e

Who reads Two-wavelength MAD phasing and radiation damage: a case study?

Author

Ana González; Frank von Delft; Robert C. Liddington; Constantina Bakolitsa

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0909-0495)

Published

2005

Language

EN

ISBN

9780909049508

Field

Physics and Astronomy (Physical Sciences)