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Differences Amid Bone Marrow and Cord Blood Hematopoietic Stem/progenitor Cell Division Kinetics by Cláudia Lobato da Silva; Raquel Gonçalves; Christopher D. Porada; João L. Ascensão; Esmail D. Zanjani; Joaquim M.S. Cabral; Graça Almeida-Porada is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Human hematopoietic stem/progenitor cells (HSC) isolated based upon specific patterns of CD34 and CD38 expression, despite phenotypically identical, were found to be functionally heterogeneous, raising the possibility that reversible expression of these antigens may occur during cellular activation and/or proliferation. In these studies, we combined PKH67 tracking with CD34/CD38 immunostaining to compare cell division kinetics between human bone marrow (BM) and cord blood (CB)‐derived HSC expanded in a serum‐free/stromal‐based system for 14 days (d), and correlated CD34 and CD38 expression with the cell divisional history. CB cells began dividing 24 h earlier than BM cells, and significantly higher numbers underwent mitosis during the time in culture. By d10, over 55% of the CB‐cells reached the ninth generation, whereas BM‐cells were mostly distributed between the fifth and seventh generation. By d14, all CB cells had undergone multiple cell divisions, while 0.7–3.8% of BM CD34^+^ cells remained quiescent. Furthermore, the percentage of BM cells expressing CD34 decreased from 60.8 ± 6.3% to 30.6 ± 6.7% prior to initiating division, suggesting that downmodulation of thi
Who reads Differences Amid Bone Marrow and Cord Blood Hematopoietic Stem/progenitor Cell Division Kinetics?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Cláudia Lobato da Silva; Raquel Gonçalves; Christopher D. Porada; João L. Ascensão; Esmail D. Zanjani; Joaquim M.S. Cabral; Graça Almeida-Porada
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)