About this Medicine article

Extrahepatic Biliary Obstruction Impairs Microvascular Perfusion and Increases Leukocyte Adhesion in Rat Liver by T A Koeppel; M Trauner; J C Baas; J C Thies; S F Schlosser; S Post; M Gebhard; C Herfarth; J L Boyer; G Otto is a Medicine article available to read on EtoBox.

To determine if disturbances of the liver microcirculation may be of pathophysiological relevance for liver damage during acute biliary obstruction, we studied the effects of bile duct ligation (BDL) on hepatic microhemodynamics and leukocyte adhesion in rat liver in vivo. Male Wistar rats were subjected to BDL for 3 days and 7 days, respectively. Sham-operated controls underwent laparotomy without BDL. After 3 days, intravital fluorescence microscopy (IVM) and hydrogen gas (H2) clearance were performed to study hepatic microvascular perfusion. Furthermore, leukocyte-endothelial cell interactions were assessed by IVM. Intercellular adhesion molecule 1 (ICAM-1) protein expression was studied by Western blot analysis and tissue immunofluorescence after 3 and 7 days, respectively. Analysis of microvascular perfusion by IVM revealed a marked impairment of sinusoidal perfusion after 3 days. Assessment of H2 clearance confirmed that overall hepatic microvascular perfusion was decreased. In addition, increased leukocyte adhesion in sinusoids and venules could be observed. A concomitant increase of ICAM-1 expression in liver tissue was also noted within the first week after BDL. Our result

Author

T A Koeppel; M Trauner; J C Baas; J C Thies; S F Schlosser; S Post; M Gebhard; C Herfarth; J L Boyer; G Otto

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Elsevier BV; Wiley; Research Square (ISSN 0270-9139)

Published

1997

Language

EN

Field

Medicine (Health Sciences)