Can I read Measuring Sulforaphane and Its Metabolites in Human Plasma: A High Throughput Method on EtoBox?

Measuring Sulforaphane and Its Metabolites in Human Plasma: A High Throughput Method by Langston-Cox, Annie; Anderson, Dovile; Creek, Darren J.; Palmer, Kirsten; Wallace, Euan M.; Marshall, Sarah A. is a Chemistry article available to read on EtoBox.

What is Measuring Sulforaphane and Its Metabolites in Human Plasma: A High Throughput Method about?

(1) Background: There is increasing understanding of the potential health benefits of cruciferous vegetables. In particular sulforaphane (SFN), found in broccoli, and its metabolites sulforaphane-glutathione (SFN-GSH), sulforaphane-cysteine (SFN-Cys), sulforaphane cysteine-glycine (SFN-CG) and sulforaphane-N-acetyl-cysteine (SFN-NAC) have potent antioxidant effects that may offer therapeutic value. Clinical investigation of sulforaphane as a therapeutic antioxidant requires a sensitive and high throughput process for quantification of sulforaphane and metabolites; (2) Methods: We collected plasma samples from healthy human volunteers before and for eight hours after consumption of a commercial broccoli extract supplement rich in sulforaphane. A rapid and sensitive method for quantification of sulforaphane and its metabolites in human plasma using Liquid Chromatography–Mass Spectrometry (LC–MS) has been developed; (3) Results: The LC–MS analytical method was validated at concentrations ranging between 3.9 nM and 1000 nM for SFN-GSH, SFN-CG, SFN-Cys and SFN-NAC and between 7.8 nM and 1000 nM in human plasma for SFN. The method displayed good accuracy (1.85%–14.8% bias) and reproducib

Who reads Measuring Sulforaphane and Its Metabolites in Human Plasma: A High Throughput Method?

Author

Langston-Cox, Annie; Anderson, Dovile; Creek, Darren J.; Palmer, Kirsten; Wallace, Euan M.; Marshall, Sarah A.

Publisher

Molecular Diversity Preservation International; MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Berlin: Springer, 1996-; Basel: 1997-; Springer Science and Business Media LLC (ISSN 1420-3049)

Published

2020

Language

EN

Field

Chemistry (Life Sciences)