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Homeobox gene HOPX is epigenetically silenced in human uterine endometrial cancer and suppresses estrogen-stimulated proliferation of cancer cells by inhibiting serum response factor by Shinichiro Yamaguchi; Kazuo Asanoma; Tomoka Takao; Kiyoko Kato; Norio Wake is a Medicine article available to read on EtoBox.

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## Abstract __HOPX (homeodomain only protein X__) is a newly identified homeobox gene whose loss of expression has been reported for several types of neoplasm. Although we found most human uterine endometrial cancers (HEC) defective in HOPX expression, genetic mutations in the __HOPX__ gene were undetectable. As is the case with several tumor suppressor genes, the promoter region of __HOPX__ is densely methylated in HEC tissue samples obtained by laser capture microdissection. __HOPX__ mRNA and protein levels were reduced in the majority of samples, and this correlated with hypermethylation of the __HOPX__ promoter. Forced expression of HOPX resulted in a partial block in cell proliferation, __in vivo__ tumorigenicity and c‐fos gene expression in HEC and MCF7 cells in response to 17β‐estradiol (E~2~) stimulation. Analysis of the serum response element (SRE) of __c‐fos__ gene promoter showed that the effect of HOPX expression is associated with inhibition of E~2~‐induced c‐fos activation through the serum response factor (SRF) motif. Knockdown of HOPX in immortalized human endometrial cells resulted in accelerated proliferation. Our study indicates that transcriptional silencing of

Who reads Homeobox gene HOPX is epigenetically silenced in human uterine endometrial cancer and suppresses estrogen-stimulated proliferation of cancer cells by inhibiting serum response factor?

Author

Shinichiro Yamaguchi; Kazuo Asanoma; Tomoka Takao; Kiyoko Kato; Norio Wake

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2009

Language

EN

Field

Medicine (Health Sciences)