Can I read Rapid and Enhanced Proteolytic Digestion Using Electric-field-oriented Enzyme Reactor on EtoBox?
Rapid and Enhanced Proteolytic Digestion Using Electric-field-oriented Enzyme Reactor by Yu Zhou; Tie Yi; Sung-Soo Park; Wayne Chadwick; Rong-Fong Shen; Wells W. Wu; Bronwen Martin; Stuart Maudsley is a Chemistry article available to read on EtoBox.
What is Rapid and Enhanced Proteolytic Digestion Using Electric-field-oriented Enzyme Reactor about?
We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5 min, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our reactor improved the overall functional analysis of lipid raft proteomes by identifying more closely functionally linked proteins and elucidated a richer set of biological processes and pathways linked to the proteins than traditional in-solution methods.
Who reads Rapid and Enhanced Proteolytic Digestion Using Electric-field-oriented Enzyme Reactor?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yu Zhou; Tie Yi; Sung-Soo Park; Wayne Chadwick; Rong-Fong Shen; Wells W. Wu; Bronwen Martin; Stuart Maudsley
- Publisher
- Elsevier ; Elsevier BV (ISSN 1874-3919)
- Published
- 2011
- Language
- EN
- Field
- Chemistry (Physical Sciences)