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Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced acute lung injury by regulating miR-424-5p/MAPK14 axis by Rui Zhang; Lina Chen; Fei Huang; Xiaorong Wang; Cuihong Li is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced acute lung injury by regulating miR-424-5p/MAPK14 axis about?

## Background: Many long non-coding rnas (lncrnas) have been suggested to play critical roles in acute lung injury (ali) pathogenesis, including lncrna nuclear enriched abundant transcript 1 (neat1). ## Objective: We aimed to further elucidate the functions and molecular mechanism of neat1 in ali. ## Methods: Human pulmonary alveolar epithelial cells (hpaepics) stimulated by lipopolysaccharide (lps) were served as a cellular model of ali. cell viability and cell apoptosis were determined by cell counting kit-8 (cck-8) assay and flow cytometry, respectively. the expression of neat1, microrna-424-5p (mir-424-5p), and mitogen-activated protein kinase 14 (mapk14) was measured by quantitative real-time polymerase chain reaction (qrt-pcr) or western blot analysis. caspase activity was determined by caspase activity kit. the inflammatory responses were evaluated using enzyme-linked immunosorbent assay (elisa). the oxidative stress factors were analyzed by corresponding kits. ## Results: Neat1 was upregulated in lps-stimulated hpaepics. neat1 knockdown weakened lps-induced injury by inhibiting apoptosis, inflammation and oxidative stress in hpaepics. moreover, mir-424-5p was a direct targe

Who reads Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced acute lung injury by regulating miR-424-5p/MAPK14 axis?

Author

Rui Zhang; Lina Chen; Fei Huang; Xiaorong Wang; Cuihong Li

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)