Can I read Chromatography of Progesterone and Its Major Metabolites in Rat Plasma Using Microbore High-performance Liquid Chromatography Columns with Conventional Injection and Detection Systems on EtoBox?

Chromatography of Progesterone and Its Major Metabolites in Rat Plasma Using Microbore High-performance Liquid Chromatography Columns with Conventional Injection and Detection Systems by R.B. Taylor; K.E. Kendle; R.G. Reid; C.T. Hung is a Chemistry article available to read on EtoBox.

What is Chromatography of Progesterone and Its Major Metabolites in Rat Plasma Using Microbore High-performance Liquid Chromatography Columns with Conventional Injection and Detection Systems about?

A separation of progesterone and its metabolites 17c~-hydroxyprogesterone, 20a-hydroxy-4-pregnen-3-one, androstenedione and testosterone by high-performance liquid chromatography with a ternary solvent system is demonstrated. It is found that using a 100 x 1 mm microbore column, higher sensitivity is obtained in the ultraviolet detection of these compounds by using conventionally sized flow cells despite the higher efficiency resulting from the use of a micro-flowcell. It is also shown that, when a non-eluting solvent is used for injection, large injection volumes do not reduce column efficiency, and a 1Cfold increase in sensitivity is obtained with a lmm column in place of one 4.6 mm in diameter. Using solid-phase extraction and concentration of the sample as pretreatment, progesterone and its hydroxylated metabolites are determined in OS-ml samples of rat plasma. The progesterone levels are compared with those obtained by radioimmunoassay.

Who reads Chromatography of Progesterone and Its Major Metabolites in Rat Plasma Using Microbore High-performance Liquid Chromatography Columns with Conventional Injection and Detection Systems?

Author

R.B. Taylor; K.E. Kendle; R.G. Reid; C.T. Hung

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1987

Language

EN

Field

Chemistry (Physical Sciences)