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This document describes a method for determining triglyceride levels. It involves an enzymatic hydrolysis of triglycerides using lipases to produce glycerol and fatty acids. The glycerol is then converted by a series of enzymatic reactions to produce hydrogen peroxide, which reacts with 4-aminophenazone and chlorophenol under catalytic influence to form a quinoneimine dye. The absorbance of this dye is then measured and is directly proportional to the triglyceride concentration in the sample. The method use

Author

Yasir Mushtaq

Language

EN