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A Method Validation for the Hemoglobin Chemical Reagent Test Strip with the Addition of Ethylenediaminetetraacetic Acid to Increase the Selectivity of This Presumptive Test for Residual Blood by Carney D. Matheson; Margaret-Ashley Veall; Lucy K. Badman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A Method Validation for the Hemoglobin Chemical Reagent Test Strip with the Addition of Ethylenediaminetetraacetic Acid to Increase the Selectivity of This Presumptive Test for Residual Blood about?

Tetramethylbenzidine based chemical reagent test strips are often used in forensic science as a presumptive test for blood. These tests are designed as urinalysis test strips and include brands such as Combur®, HENSOTest®, Hemastix®, MultiStix® and Chemstrip®. They are used because they are simple to apply, stable, temperature tolerant and cost effective. The addition of a chelating agent, ethylenediaminetetraacetic acid increases the selectivity of this presumptive test for blood. This is a method validation for the hemoglobin chemical reagent test strip with EDTA. A range of substances, metal compounds, chemical solutions, blood and mixtures were tested in this method validation. The chelation with EDTA successfully prevented non-blood (false) positive results from all the substances tested and consistently produced a positive result for blood on a variety of surfaces. This study has shown that this method is capable of discriminating a blood stain on copper metal surfaces and eliminate the positive results generated by clean-up solutions such as hydrogen peroxide, which usually produce a positive result for most other presumptive tests for blood. This modified method is a simple

Who reads A Method Validation for the Hemoglobin Chemical Reagent Test Strip with the Addition of Ethylenediaminetetraacetic Acid to Increase the Selectivity of This Presumptive Test for Residual Blood?

Author

Carney D. Matheson; Margaret-Ashley Veall; Lucy K. Badman

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)