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Hybrid Chitosan/gelatin/nanohydroxyapatite Scaffolds Promote Odontogenic Differentiation of Dental Pulp Stem Cells and in Vitro Biomineralization by Georgia Vagropoulou; Maria Trentsiou; Anthie Georgopoulou; Eleni Papachristou; Oleg Prymak; Aristeidis Kritis; Matthias Epple; Maria Chatzinikolaidou; Athina Bakopoulou; Petros Koidis is a Dentistry article available to read on EtoBox.
What is Hybrid Chitosan/gelatin/nanohydroxyapatite Scaffolds Promote Odontogenic Differentiation of Dental Pulp Stem Cells and in Vitro Biomineralization about?
## Objective: Hybrid chitosan/gelatin/nanohydroxyapatite (cs/gel/nha) scaffolds have attracted considerable interest in tissue engineering (te) of mineralized tissues. the present study aimed to investigate the potential of cs/gel/nha scaffolds loaded with dental pulp stem cells (dpscs) to induce odontogenic differentiation and in vitro biomineralization. ## Methods: Cs/gel/nha scaffolds were synthesized by freeze-drying, seeded with dpscs, and characterized with flow cytometry. scanning electron microscopy (sem), live/dead staining, and mtt assays were used to evaluate cell morphology and viability; real-time pcr for odontogenesis-related gene expression analysis; sem-eds (energy dispersive x-ray spectroscopy), and x-ray diffraction analysis (xrd) for structural and chemical characterization of the mineralized constructs, respectively. ## Results: Cs/gel/nha scaffolds supported viability and proliferation of dpscs over 14 days in culture. gene expression patterns indicated pronounced odontogenic shift of dpscs, evidenced by upregulation of dspp, bmp-2, alp, and the transcription factors runx2 and osterix. sem-eds showed the production of a nanocrystalline mineralized matrix inside
Who reads Hybrid Chitosan/gelatin/nanohydroxyapatite Scaffolds Promote Odontogenic Differentiation of Dental Pulp Stem Cells and in Vitro Biomineralization?
It is typically read by researchers, students, and practitioners in Dentistry.
- Author
- Georgia Vagropoulou; Maria Trentsiou; Anthie Georgopoulou; Eleni Papachristou; Oleg Prymak; Aristeidis Kritis; Matthias Epple; Maria Chatzinikolaidou; Athina Bakopoulou; Petros Koidis
- Publisher
- Elsevier BV
- Published
- 2020
- Language
- EN
- Field
- Dentistry (Health Sciences)