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Skin Cells Undergo Asynthetic Fission to Expand Body Surfaces in Zebrafish by Keat Ying Chan; Ching-Cher Sanders Yan; Hsiao-Yuh Roan; Shao-Chun Hsu; Tzu-Lun Tseng; Chung-Der Hsiao; Chao-Ping Hsu; Chen-Hui Chen is a Physics and Astronomy article available to read on EtoBox.

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As an animal's surface area expands during development, skin cell populations must quickly respond to maintain sufficient epithelial coverage. Despite much progress in understanding of skin cell behaviours in vivo, it remains unclear how cells collectively act to satisfy coverage demands at an organismic level. Here we created a multicolour cell membrane tagging system, palmskin, to monitor the entire population of superficial epithelial cells (SECs) in developing zebrafish larvae. Using time-lapse imaging, we found that many SECs readily divide on the animal body surface; during a specific developmental window, a single SEC can produce a maximum of four progeny cells over its lifetime on the surface of the animal. Remarkably, EdU assays, DNA staining and hydroxyurea treatment showed that these terminally differentiated skin cells continue splitting despite an absence of DNA replication, causing up to 50% of SECs to exhibit reduced genome size. On the basis of a simple mathematical model and quantitative analyses of cell volumes and apical surface areas, we propose that 'asynthetic fission' is used as an efficient mechanism for expanding epithelial coverage during rapid growth. Fur

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Author

Keat Ying Chan; Ching-Cher Sanders Yan; Hsiao-Yuh Roan; Shao-Chun Hsu; Tzu-Lun Tseng; Chung-Der Hsiao; Chao-Ping Hsu; Chen-Hui Chen

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Physics and Astronomy (Physical Sciences)