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Nanomolar Derivatizations with 5-Carboxyfluorescein Succinimidyl Ester for Fluorescence Detection in Capillary Electrophoresis by Shung Kei Lau; Fabian Zaccardo; Michael Little; Peter Banks is a Chemistry article available to read on EtoBox.

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The ability of 5-carboxyfluorescein succinimidyl ester (CFSE) to derivatize amino acids at very low concentrations has been investigated. CFSE can derivatize nanomolar concentrations of amino acids, which represents a 1000-fold improvement compared to fluorescein isothiocyanate (FITC). Although the reactive probe is not fluorogenic, few hydrolysis products result from derivatization, leading to relatively clean electropherograms compared to FITC derivatization. Derivatizations of g-aminobutyric acid suggest that CFSE may be a practical amine-reactive fluorescent probe for the quantification of neurotransmitters in microdialysates.

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Author

Shung Kei Lau; Fabian Zaccardo; Michael Little; Peter Banks

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)