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This document describes the procedures for performing paper chromatography to separate pigments. Paper chromatography works by partitioning compounds between a stationary cellulose paper phase and a mobile liquid phase. Samples are prepared by grinding leaves and extracting with ethanol. The samples are spotted onto chromatography paper and developed in a hexane/ethyl acetate solvent mixture. The Rf values of separated spots are calculated. The objectives of understanding paper chromatography principles and

Author

Syaf Aronz

Language

EN