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Can I read Human Microvascular Endothelial Cells Express Integrin-related Complexes That Mediate Adhesion to the Extracellular Matrix on EtoBox?
Human Microvascular Endothelial Cells Express Integrin-related Complexes That Mediate Adhesion to the Extracellular Matrix by Yao-Fen Cheng; Randall H. Kramer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Human Microvascular Endothelial Cells Express Integrin-related Complexes That Mediate Adhesion to the Extracellular Matrix about?
Microvascular endothelial cells (MEC) must use a set of surface receptors to adhere not only to the vascular basement membrane but, during angiogenic stimulation, to the interstitium. We examined how cultured MEC isolated from human foreskin interact with their subendothelial matrix. MEC were able to attach to diverse extracellular matrix proteins, including fibronectin (Fn), vitronectin (Vn), laminin (Ln), type I and IV collagen, as well as to fibrinogen and gelatin. Adhesion to Fn, but not to larninin or collagens, was specifically blocked in the presence of Arg-Gly-Asp (RGD)-containing peptides. When surface radioiodinated MEC were solubilized and subjected to affinity chromatography on Fn-Sepharose columns, two polypeptides of 150 and 125 kD, corresponding to the integrin heterodimer a5pl, were identified. MEC also express a complex of 150 (a) and 95 kD (p,) that is related to the Vn receptor. lrnmunofluorescent staining of MEC cultures with antibodies to the integrin p1 subunit demonstrated receptors on the basolateral surface at focal adhesion plaques that co-localized with vinculin and with Fn-positive matrix fibers. Occasionally, antibodies to the Vn receptor stained the vi
Who reads Human Microvascular Endothelial Cells Express Integrin-related Complexes That Mediate Adhesion to the Extracellular Matrix?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yao-Fen Cheng; Randall H. Kramer
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 1989
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)