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Separation and Determination of Podophyllum Lignans by Micellar Electrokinetic Chromatography by Shuhui Liu; Xuan Tian; Xingguo Chen; Zhide Hu is a scholarly article available to read on EtoBox.

What is Separation and Determination of Podophyllum Lignans by Micellar Electrokinetic Chromatography about?

A micellar electrokinetic chromatography method was established for the quantitative analysis of seven podophyllum lignans in Podophyllum emodi Wall. var. chinesis sprague. The optimum buffer system was 10 mM NaH PO -5 mM 2 4 borate-100 mM sodium dodecylsulfate-30% isopropanol (pH 7.20). Voltage was 18 kV and detection at 214 nm. The second derivative chromatogram was used to determine a low-content component and those not fully separated from adjacent ones. The RSD values of migration times and peak areas were ,2.2 and ,5.5%, respectively. The effects of several CE parameters on the resolutions were studied systematically.

Author

Shuhui Liu; Xuan Tian; Xingguo Chen; Zhide Hu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2001

Language

EN