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Cloning of the fructan biosynthesis pathway of Jerusalem artichoke by Ingrid M. Van Der Meer; Andries J. Koops; Johanna C. Hakkert; Arjen J. Van Tunen is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary To study the regulation of fructan synthesis in plants, we isolated two full‐size cDNA clones encoding the two enzymes responsible for fructan biosynthesis in Jerusalem artichoke ( __Helianthus tuberosus__ ): 1‐sucrose:sucrose fructosyl transferase (1‐SST) and 1‐fructan:fructan fructosyl transferase (1‐FFT). Both enzymes have recently been purified to homogeneity from Jerusalem artichoke tubers (Koops and Jonker (1994) __J. Exp. Bot.__ 45, 1623–1631; Koops and Jonker (1996) __Plant Physiol.__ 110, 1167–1175) and their amino acid sequences have been partially determined. Using RT–PCR and primers based on these sequences, specific fragments of the genes were amplified from tubers of Jerusalem artichoke. These fragments were used as probes to isolate the cDNAs encoding 1‐SST and 1‐FFT from a tuber‐specific λZAP library. The deduced amino acid sequences of both cDNAs perfectly matched the sequences of the corresponding purified proteins. At the amino acid level, the cDNA sequences showed 61% homology to each other and 59% homology to tomato vacuolar invertase. Based on characteristics of the deduced amino acid sequence, the first 150 bp of both genes encode a putative vacuol

Who reads Cloning of the fructan biosynthesis pathway of Jerusalem artichoke?

Author

Ingrid M. Van Der Meer; Andries J. Koops; Johanna C. Hakkert; Arjen J. Van Tunen

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)