Can I read Calcium-dependent K current in plasma membranes of dermal cells of developing bean cotyledons on EtoBox?

Calcium-dependent K current in plasma membranes of dermal cells of developing bean cotyledons by W.-H. ZHANG; N. A. WALKER; J. W. PATRICK; S. D. TYERMAN is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Calcium-dependent K current in plasma membranes of dermal cells of developing bean cotyledons about?

## ABSTRACT In developing seeds of bean (__Phaseolus vulgaris__ L.), phloem‐imported assimilates (largely sucrose and potassium) are released from coats to seed apoplasm and subsequently retrieved by the dermal cell complexes of cotyledons. To investigate the mechanisms of K^+^ uptake by the cotyledons, protoplasts of dermal cell complexes were isolated and whole‐cell currents across their plasma membranes were measured with the patch‐clamp technique. A weakly rectified cation current displaying a voltage‐dependent blockade by external Ca^2+^ and acidic pH, dominated the conductance of the protoplasts. The __P haseolus v ulgaris__ Cotyledon Dermal‐cell pH and Calcium‐dependent Cation Conductance (Pv‐CD‐pHCaCC) was highly selective for K^+^ over Ca^2+^ and Cl^–^. For K^+^ current through Pv‐CD‐pHCaCC a sigmoid shaped current–voltage (__I__–__V__) curve was observed with negative conductance at voltages between −200 and −140 mV. This negative K^+^ conductance was Ca^2+^ dependent. With other univalent cations (Na^+^, Rb^+^, NH~4~^+^) the currents were smaller and were not Ca^2+^ dependent. Reversal potentials remained constant when external K^+^ was substituted with these cations, su

Who reads Calcium-dependent K current in plasma membranes of dermal cells of developing bean cotyledons?

Author

W.-H. ZHANG; N. A. WALKER; J. W. PATRICK; S. D. TYERMAN

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0140-7791)

Published

2004

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)