About this Biochemistry, Genetics and Molecular Biology article
Dominance of recombinant cotton leaf curl Multan-Rajasthan virus associated with cotton leaf curl disease outbreak in northwest India by Biswas, Kajal Kumar; Bhattacharyya, Utpal Kumar; Palchoudhury, Supratik; Balram, Nenavath; Kumar, Anil; Arora, Rupesh; Sain, Satish Kumar; Kumar, Pradeep; Khetarpal, Ravi K.; Sanyal, Amitava; Mandal, Pranab Kumar; Melcher, Ulrich is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
Cotton leaf curl disease (CLCuD), caused by whitefly (__Bemisiatabaci__) transmitted single-stranded DNA viruses belonging to the Genus, __Begomovirus__ (family, __Geminiviridae__) in association with satellite molecules; is responsible for major economic losses in cotton in three northwest (NW) Indian states Haryana, Punjab, and Rajasthan. Annual CLCuD incidences during 2012 to 2014 were estimated to be 37.5%, 63.6%, and 38.8% respectively. Cotton leaves were collected from symptomatic plants annually for three years and subjected to DNA isolation, followed by rolling circle amplification (RCA), cloning, and DNA sequencing of apparently full-length begomoviral genomes and associated betasatellites and alphasatellites. Among the thirteen CLCuD-begomoviral genomes recovered, eight were identified as __Cotton leaf curl Multan virus__-Rajasthan (CLCuMuV-Ra), one as -Pakistan (PK) and another as -Faisalabad (Fai), whereas, three were as __Cotton leaf curl Kokhran virus__-Burewala (CLCuKoV-Bu), indicating that CLCuMuV-Ra was the most prevalent begomovirus species. Five of the eight CLCuMuV-Ra sequences were found to be recombinants. The CLCuMuV-Ra- associated satellites consisted of __C
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- Author
- Biswas, Kajal Kumar; Bhattacharyya, Utpal Kumar; Palchoudhury, Supratik; Balram, Nenavath; Kumar, Anil; Arora, Rupesh; Sain, Satish Kumar; Kumar, Pradeep; Khetarpal, Ravi K.; Sanyal, Amitava; Mandal, Pranab Kumar; Melcher, Ulrich
- Publisher
- Public Library of Science; San Francisco CA: Public Library of Science; Publons; ScienceOpen; Public Library of Science (PLoS) (ISSN 1932-6203)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)