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Ellagic Acid from Acorn Fringe by Enzymatic Hydrolysis and Combined Effects of Operational Variables and Enzymes on Yield of the Production by Wen Huang; Hai Niu; Zhenshan Li; Lulu Li; Wang Wang is a Engineering article available to read on EtoBox.

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The individual effects of three different enzyme types -one single enzyme (ellagitannin acyl hydrolase) and two combinations of enzymes (ellagitannin acyl hydrolase-b-glucosidase-polyphenol oxidase and ellagitannin acyl hydrolase-cellulase-xylanase) -on ellagic acid yield, combined with other process parameters -enzyme concentration, hydrolysis time, particle size and solid-to-liquid ratio -were evaluated by response surface methodology. The selection of the enzymes for the study was based on preliminary experiments that showed higher increments in ellagic acid yield. The quantitative parameters studied were enzyme concentration (0.1, 0.45, 2 w/w or %), solid-to-liquid ratio (0.05, 0.15, 0.2), particle size (220, 445, 900 lm) and hydrolysis time (60, 89, 132 min). Experimental data for ellagic acid yield obtained with a single enzyme and two combination enzymes correlated very well with process parameters (P < 0.0001), resulting in models with high coefficient of determination for ellagic acid yield (r 2 = 0.9636). The combinations of enzymes appeared more effective for ellagic acid production than the single enzyme did. The yield of ellagic acid from non-heat-treated acorn fringe

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Author

Wen Huang; Hai Niu; Zhenshan Li; Lulu Li; Wang Wang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0960-8524)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)