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Third-generation Superoxide Anion Sensor Based on Superoxide Dismutase Directly Immobilized by Sol–gel Thin Film on Gold Electrode by Junwei Di; Shuping Bi; Ming Zhang is a Engineering article available to read on EtoBox.
What is Third-generation Superoxide Anion Sensor Based on Superoxide Dismutase Directly Immobilized by Sol–gel Thin Film on Gold Electrode about?
A third-generation biosensor for superoxide anion (O 2 •-) was developed based on superoxide dismutase (SOD) immobilized by thin silica-PVA sol-gel film on gold electrode surface. A rapid and direct electron transfer of SOD in the thin sol-gel film at the gold electrode was realized without any mediators or promoters. The characterization of the SOD electrodes showed a quasi-reversible electrochemical redox behavior with a formal potential of 80 + 5 mV (versus SCE) in 50 mmol l -1 phosphate buffer solution (PBS), pH 7.0. The heterogeneous electron transfer rate constant was evaluated to be about 2.1 s -1 . The anodic and cathodic transfer coefficients are 0.6 and 0.4, respectively. Based on biomolecular recognition for specific reactivity of SOD toward O 2 •-, the SOD electrode was applied to a sensitive and selective measurement of O 2 •-with the low operation potential (-0.15 V versus SCE) in phosphate buffer solution, pH 7.0. The amperometric response was proportional to O 2 •-concentration in the range of 0.2-1.6 mol l -1 and the detection limit was 0.1 mol l -1 at a signal-to-noise ration of 3. The preparation of SOD electrode is easy and simple. The uniform porous structure o
Who reads Third-generation Superoxide Anion Sensor Based on Superoxide Dismutase Directly Immobilized by Sol–gel Thin Film on Gold Electrode?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Junwei Di; Shuping Bi; Ming Zhang
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2004
- Language
- EN
- Field
- Engineering (Physical Sciences)