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Elevated levels of prostate‐specific antigen (PSA) in prostate cancer cells expressing mutant p53 is associated with tumor metastasis by Sean Downing; Clare Bumak; Sheri Nixdorf; Kim Ow; Pamela Russell; Paul Jackson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The underlying basis for rising levels of prostate‐specific antigen (PSA) in prostate cancer is not fully understood, but attention has turned to the possibility that loss of normal p53 function might be directly involved. We have investigated the relationship between p53 function and PSA expression using in vitro and in vivo approaches. Three prostate cancer–derived p53 mutants (F134L, M237L, R273H) were introduced into LNCaP prostate cancer cells and stable transfectants established. Expression of mutant p53 was demonstrated by Western blot analysis, inactivation of wtp53 function, and a loss of p53‐dependent responses to DNA damage induced by UV‐irradiation and cisplatin. Levels of PSA mRNA and secreted protein were determined by RT‐PCR and Western blotting, respectively. Serine protease activity was assessed using an esterase assay. In vivo effects of mutant p53 expression were examined after orthotopic implantation into prostates of nude mice. Expression of all p53 mutants was associated with elevated PSA mRNA and secreted PSA protein. In a representative line, mutant p53 was also associated with increased PSA protease‐like activity compared with a control line exp

Who reads Elevated levels of prostate‐specific antigen (PSA) in prostate cancer cells expressing mutant p53 is associated with tumor metastasis?

Author

Sean Downing; Clare Bumak; Sheri Nixdorf; Kim Ow; Pamela Russell; Paul Jackson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)