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Two α-tubulin genes of Aspergillus nidulans encode divergent proteins by Parul Doshi; Cynthia A. Bossie; John H. Doonan; Gregory S. Mays; N. Ronald Morris is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We have isolated and analyzed the tubA and tubB alpha-tubulin genes of Aspergillus nidulans. The nucleotide sequences of these genes predict polypeptides of 447 amino acids for tubA and 450 for tubB. The predicted amino acids sequences exhibit 28% divergence between the two polypeptides. This is the second known case of such high divergence between alpha-tubulins within the same species. The tubB gene is unique in that it codes for an extra glycine residue between what are usually the second and third amino acids. RNA blot analysis demonstrates that the tubA and tubB transcripts are each 1.8 kb long. The level of tubA transcript remains the same throughout the cell cycle. The level of tubB transcript does not change at any particular stage in the cell cycle but increases continuously during spore germination. The tubA gene was previously mapped to linkage group eight, and we have now mapped the tubB gene to linkage group four. Gene disruption in heterokaryons suggests that the phenotypic consequences of disruption are different for the tubA and tubB genes. Molecular disruption of tubA results in a block in nuclear division whereas in tubB it gives rise to abnormal cell and nuclear

Who reads Two α-tubulin genes of Aspergillus nidulans encode divergent proteins?

Author

Parul Doshi; Cynthia A. Bossie; John H. Doonan; Gregory S. Mays; N. Ronald Morris

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)